PMID- 11733287
DA - 20011204
DCOM- 20020124
IS - 1069-6563
VI - 8
IP - 12
DP - 2001 Dec
TI - Neurotensin analog NT69L induces rapid and prolonged hypothermia after hypoxic ischemia.
PG - 1115-21 AB - OBJECTIVE: To determine whether the neurotensin analog NT69L, administered systemically, could induce mild brain hypothermia after asphyxial cardiac arrest (ACA) in rats. METHODS: The study design was experimental, blinded, randomized, and approved by the animal use committee. All rats had continuous monitoring of brain temperature and sustained 8 minutes of ACA, resuscitation, and either saline or NT69L intravenously after return of spontaneous circulation (ROSC). Rats surviving 14 days after ACA had a neurological deficit score (NDS) and a Morris Water Maze (MWM) test. RESULTS: Seven of eight rats in each group survived 14 days. Brain temperature was less than 35 degrees C 13.1 +/- 3 minutes (mean +/- standard deviation) after NT69L vs controls that remained 37.5 degrees C at the same ambient temperature (p < 0.05 ANOVA). The NT69L group remained below 35 degrees C for 300 +/- 100 minutes while the controls remained at 37.5 +/- 0.5 degrees C. The NDS in the NT69L rats was 3 +/- 3% vs controls 26 +/- 8% (p < 0.05, Kruskal-Wallis, 0% = normal, 100% = brain dead). The NT69L rats performed better on the MWM vs the controls (22 +/- 8 sec vs 45 +/- 26 sec, respectively, p < 0.05 ANOVA). CONCLUSIONS: NT69L induced rapid and prolonged mild brain hypothermia after ACA in this rat model and reduced neurological deficits.
AD - Department of Emergency Medicine, University of North Carolina at Chapel
[emd]
FAU - Katz, L M
AU - Katz LM
FAU - Wang, Y
AU - Wang Y
FAU - McMahon, B
AU - McMahon B
FAU - Richelson, E
AU - Richelson E
LA - eng
PT - Journal Article
CY - United States
TA - Acad Emerg Med
JID - 9418450
RN - 0 (Peptide Fragments)
RN - 0 (neurotensin 69L)
RN - 39379-15-2 (Neurotensin)
SB - IM
MH - Analysis of Variance
MH - Animal
MH - Blood Gas Analysis
MH - Cardiopulmonary Resuscitation
MH - Comparative Study
MH - Disease Models, Animal
MH - Heart Arrest/complications/therapy
MH - Hypothermia/*chemically induced/physiopathology
MH - Hypoxia-Ischemia, Brain/etiology/*prevention & control
MH - Infusions, Intravenous
MH - Male
MH - Neurotensin/administration & dosage/*analogs & derivatives/*pharmacology
MH - Peptide Fragments/administration & dosage/*pharmacology
MH - Probability
MH - Random Allocation
MH - Rats
MH - Rats, Sprague-Dawley
MH - Reference Values
MH - Sensitivity and Specificity
MH - Time Factors
EDAT- 2001/12/06 10:00
MHDA- 2002/01/25 10:01
URLF- http://www.aemj.org/cgi/content/full/8/12/1115
URLS- http://www.aemj.org/cgi/content/abstract/8/12/1115
PST - ppublish
SO - Acad Emerg Med 2001 Dec;8(12):1115-21.